Jeffrey Givens, 51, of Murfreesboro was southbound on Interstate 71 in Gallatin County, Ky., when the truck he was driving with an attached horse trailer ran off the left side of the road, authorities told Cincinnati media outlets.
The truck and trailer jackknifed across the interstate and hit a guardrail with the truck landing on its roof.
Givens, who died at the scene, and passenger Tim "Lightning" Taylor, 53, of La Vergne were pinned in the truck. Taylor suffered a broken shoulder and ribs and was taken to a Cincinnati hospital, WKRN-TV (Channel 2) of Nashville said.
Three of the horses were seriously injured and euthanized at the scene, WCPO-TV of Cincinnati reported. They were Maxximize, Incredible and Trigger on the Prowl, unidentified sources told WKRN-TV.
Two horses survived, one requiring stitches, and were taken to a Cincinnati-area stable.
"The hard part is that you have to wait for the vet to get here to sedate the horses," Glencoe (Ky.) Fire Chief Bud Webster told WLWT-TV of Cincinnati. "They're pretty emotional. They can hurt the first responders if you get them free before they're sedated."
Givens, owner of Jeff Givens Stables, was well known for training horses owned by Jo Ann and Sue Ann Dowell, a mother-daughter team from Powell, Ohio.